This step is required for all new users regardless of which scope or service you plan to use. We require a registration form be filled out on every person utilizing resources (both equipment and staff) in all CISR facilities. Information from the registration form allows us to set up a user account for you and grant security access to the equipment. You can download a PDF version to fill out by hand or download the RTF version and email it as an attachment. Once you have the completed registration form (including your PI's authorization signature), return it to any of the CISR staff in 742 Light Hall, T3208 MCN, T-2216 MCN or 4155 MRBIII.
Visit the equipment pages of our web site and read the instrument descriptions to determine which microscope you should use. If you are still not sure which scope best meets your needs, feel free to visit the Staff page. You can contact any of us by phone, email, or even in person - we'd love to give you a brief tour of the facilities and discuss your research opportunities. We will help you figure it out!
For confocal microscopes: at least two, 2-hour training sessions are required before you can use the microscopes independently; electron and widefield microscopes: at least one, 2-hour training session is scheduled, but times may vary.
Of special note on the TEM and SEM microscopes: new users are restricted to use during normal business hours (Monday through Friday 9:00 am until 6:00 pm). Use on weekends or after hours is limited to those who have extensive experience in their operation and have undergone additional training in how to shut down the microscope in case of a problem. Since no staff member is around after hours to help in an emergency, this policy is designed to avoid costly damage to the microscopes.
Submitted specimens must be accompanied by a Research Electron Microscopy Resource work requisition. All samples must now go through the EM online laboratory information management system located here. Specimens should be fixed prior to submission and kept refrigerated until submitted. EM fixative and wash buffer can be obtained from the refrigerator in the Processing Lab (T-3208 MCN).
|*RATE / UNIT|
|Confocal, scope time||$45.00 / hour|
|Widefield, scope time||$35.00 / hour|
|Super Res, scope time||$20.00 / hour|
|Multiphoton Electrophys, scope time||$20.00 / hour|
|TEM, scope time||$100.00 / hour|
|ESEM, scope time||$50.00 / hour|
|Nikon Center of Excellence|
|Nikon Spinning Disk, scope time||$15.00 / hour|
|Nikon SIM Super Resolution, scope time||$15.00 / hour|
|Nikon STORM Super Resolution, scope time||$20.00 / hour|
|embed (Spurr's)||$45.00 / capsule|
|thick section (routine)||$50.00 / block|
|thick section (non-routine) > 1 hour process||$60.00 / hour|
|thin section (routine)||$100.00 / block|
|thin section (non-routine) > 2 hour process||$60.00 / hour|
|negative stain, material only||$6.00 / grid|
|Immunolabel, material only||$20.00 / kit|
|ESEM prep, material only||$30.00 / stub|
|TEM grid box||$5.25 / each|
|Staff Review - thin section EM||$60.00 / grid|
|Imaging for hire, training, protracted help||$60.00 / hour|
The resource staff are happy to answer questions about our services and will help you plan your experiments. If you have questions please feel free to contact any member of the resource staff.