The Vanderbilt University Nikon Center of Excellence (NCoE) is managed and maintained by the CISR. The NCoE enables researchers across the scientific spectrum, including faculty, post-docs, as well as graduate and undergraduate student researchers access to Nikon’s most advanced microscopy and imaging platforms. The NCoE will further support Vanderbilt’s Basic Sciences community by offering training courses, including basic and advanced light microscopy techniques, quantitative image analysis, and practical hands-on training.
The facility, located in T-2216 MCN, houses three microscopes and two offline processing workstations. Instrumentation includes a spinning disk confocal microscope adapted for live cell imaging and additionally equipped with a steerable high-energy laser for simultaneous, photo -activation, -conversion, -bleaching, and targeted photo-damage. Additionally, an upgraded system for high-speed stochastic optical reconstruction microscopy (STORM), provides resolving powers up to 10x that of traditional light microscopes. Finally, Nikon’s flagship structured illumination microscope (SIM), allows for super-resolution imaging of samples without stringent preparation protocols and in live cells through the use of the 2D, 3D, and TIRF (total internal reflection fluorescence) modalities. The center provides two high-end analysis workstations with a full complement of software for advanced image analysis and 3D rendering including 2D/3D deconvolution, segmentation, and tracking (including single particle tracking), as well as FRAP (fluorescence recovery after photo-bleaching), to name a few. All of these state-of-the-art systems are housed in a newly constructed facility designed to minimize temperature fluctuations and dust contamination. Further, the facility has a dedicated tissue culture prep room complete with culture hood, 37C incubator, and refrigeration for user’s live cells and reagents.
All CISR managed equipment is limited to registered users only. Please Click Here for more information on the registration process.