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Registration is required for log in access to user only areas of this website such as scheduling and sample submission. Training by CISR staff is required for access to and independent use of any CISR equipment. Click the register button below to obtain more information for New Users and for access to registration forms.
 
Charges: $80 per hour
Note: To use the microscope you must have an active Account with the Cell Imaging Shared Resource and reserve time using our on-line Scheduling calendar. Contact Denny, Mary, or Jay to arrange training.


Location

Download / View the:
   CM-12 TEM Quickstart Guide.
   AMT Digital Acquisition Camera Quickstart Guide.
Why Use This Microscope?
The Philips CM-12, is a high resolution transmission electron microscope with eucentric goniometer state and Gatan cryotransfer stage. It provides much higher resolution (ability to view smaller objects) than that provided by light microscopy. There is a side mounted 2k x 2k high resolution CCD camera system for the microscope to facilitate digital image collection and analysis.
Research Techniques:
The laboratory is fully equipped to carry out numerous aspects of "routine" as well as specialized electron microscopy. Included in the specialized services available through the resource are preparative techniques, such as:
  • Embedding and sectioning
  • Negative staining
  • Immunolabeling
  • Enzyme cytohemistry
  • Critical point drying for scanning electron microscopy
  • Image quantification
  • Image processing
  • Pattern analysis
In addition, collaboration between the resource and the Engineering school provide access to intermediate voltage electron microscopy, scanning electron microscopy, and X-ray microanalysis. The director has extensive knowledge of all of these techniques.
Additional Equipment
  • Leica Ultracut microtomes
  • Cressington 108 sputter coater
  • Slam and plunge freezing equipment.
In addition, the School of Engineering has a Philips CM-20 Intermediate Voltage Electron microscope with STEM capability and a Hitachi X-650 scanning electron microscope with both EDS and WDS capabilities. These instruments are available to all University users and guidance and training in use of the equipment for biological samples is provided by the Electron Microscopy Resource.