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Registration is required for log in access to user only areas of this website such as scheduling and sample submission. Training by CISR staff is required for access to and independent use of any CISR equipment. Click the register button below to obtain more information for New Users and for access to registration forms.
 
Charges: $25.00 per hour
Note: To use the microscope you must have an active Account with the Cell Imaging Shared Resource and reserve time using our on-line Scheduling calendar. Contact Bob, Jenny, Sean, or Sam to arrange training.


Location
  • MRBIV 10465


Download / View Quickstart Guide.
Why Use This Microscope?
This microscope is for super resolution imaging that can reach resolutions of 120 nm in XY and 300 nm in Z. The OMX is well suited for viewing samples on sealed, inverted slides or in 35 mm dishes with #1.5 coverglass (MatTek). All slides MUST be sealed, clean, and dry prior to place on the stage. The OMX has its own set of oils to precisely match the refractive index of your sample.
Camera:
  • sCMOS camera at 512x512 for 3D-SIM
Imaging Modes:
  • Structured Illumination Microscopy
  • Conventional fluorescence microscopy
  • DIC
Fluorescence Filters: (excitation/emission)
  • 405 laser (Near UV)
  • 488 laser (GFP, FITC, Cy2, Alexa488)
  • 568 laser (Cherry, TRITC, Cy3, Alexa568)
  • 642 laser (Far Red, Cy5, Alexa647)
Objective Lenses:
  • 60x Plan-Apochromat N/1.42 NA oil
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