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Registration is required for log in access to user only areas of this website such as scheduling and sample submission. Training by CISR staff is required for access to and independent use of any CISR equipment. Click the register button below to obtain more information for New Users and for access to registration forms.
Charges: $35 per hour
Note: To use the microscope you must have an active Account with the Cell Imaging Shared Resource and reserve time using our on-line Scheduling calendar. Contact Carol Ann, Bob, Mary, Sean, or Sam to arrange training.


Locations
  • 4155 MRBIII
  • 742A Light Hall


Download / View Quickstart Guide.
Why Use This Microscope?
This microscope is well suited for viewing samples on sealed, inverted slides or in 35mm dishes with a #1.5 coverglass (Matek) compatible with the inverted format. All slides MUST be sealed, clean and dry prior to placing on the stage.
Imaging Modes:
  • Laser scanning fluorescence and DIC (Nomarski)
  • 3-D "Z-series"
  • Time-series
Fluorescence Filters: (excitation/emission)
  • Blue/Green (457,488,514nm for blue, green, yellow dyes,
        e.g., CFP,YFP,GFP, Cy-2, FITC-like dyes)
  • Green/Red (543nm for Cy-3, Texas Red, or rhodamine-like dyes)
  • Red/Far-Red (633 nm for far-red dyes like Cy-5)
Objective Lenses:
  • 5x/.30 Plan-Neofluar (4155 MRBIII only)
  • 10x / 0.30 Plan Neofluar
  • 20x / 0.75 Plan-Apochromat
  • 40x / 1.30 Plan-NEOFLUAR OIL
  • 63x / 1.40 Plan-APOCHROMAT OIL
  • 100x / 1.30 Plan-NEOFLUAR OIL