Charges: $35 per hour
Note: To use the microscope you must have an active Account with the Cell Imaging Shared Resource and reserve time using our on-line Scheduling calendar. Contact Carol Ann, Bob, Mary, Sean, or Sam to arrange training.
Location
Download / View Quickstart Guide.
Note: To use the microscope you must have an active Account with the Cell Imaging Shared Resource and reserve time using our on-line Scheduling calendar. Contact Carol Ann, Bob, Mary, Sean, or Sam to arrange training.
Location
- 714A Light Hall
Download / View Quickstart Guide.
Why Use This Microscope?
This microscope is well suited for viewing samples upright on slides using a SEALED #1.5 coverglass. This instrument is not to be used with dishes or open containers of any sort - these formats should be used on the Leica inverted microscope. All slides MUST be sealed, clean and dry prior to placing on the stage.
This microscope is well suited for viewing samples upright on slides using a SEALED #1.5 coverglass. This instrument is not to be used with dishes or open containers of any sort - these formats should be used on the Leica inverted microscope. All slides MUST be sealed, clean and dry prior to placing on the stage.
Detector / Cameras:
- Princeton Instruments MicroMax CCD camera for low-light, quantitative fluorescence.
- Photometrics CoolSnap digital camera for color brightfield images, can also be used for multi-color fluorescence, but less sensitive than the MicroMax and not quantitative.
- Explanation of size of digital images taken by CCD cameras.
- Two 35mm film cameras with Zeiss computerized controller
Imaging Modes:
- Wide-field (conventional) fluorescence
- Transmitted light (including phase and DIC)
Fluorescence Filters: (excitation/emission)
- UV/Blue (365 nm for blue dyes such as DAPI and Hoechst)
- Blue/Green BP (470 nm for green labels such as Cy-2 and FITC-like dyes)
- Green/Red (546 nm for Cy-3, Texas Red, or rhodamine-like dyes)
Objective Lenses:
- 5x / 0.15 Plan-NEOFLUAR
- 10x / 0.30 Plan Neofluar Ph1
- 20x / 0.50 Plan-Neofluar Ph2
- 40x / 1.30 Plan-Neofluar oil DIC
- 63x / 1.40 Plan-Apochromat oil DIC
- 100x / 1.40 Plan-Apochromat oil DIC
