lockCISRLogin
You must be registered!
Registration is required for log in access to user only areas of this website such as scheduling and sample submission. Training by CISR staff is required for access to and independent use of any CISR equipment. Click the register button below to obtain more information for New Users and for access to registration forms.
 
Who We Are
Scheduling Reservations
CISR News
Equipment Status
Light Hall
742A - LSM 510 Meta Confocal Not reserved or in use.
742B - LSM 510 Confocal (invert) Not reserved or in use.
742C - LSM 710 Not reserved or in use.
708 - Axiophot Not reserved or in use.
708 - Leica Conventional (invert) Not reserved or in use.
708 - AZ100M Not reserved or in use.
842 - LSM5 Live Not reserved or in use.
MRBIII
4155 - LSM 510 Confocal (invert) Not reserved or in use.
7149 - LSM 510 Confocal (upright) Not reserved or in use.
MRBIV
10434 - FV1000 Confocal (invert) Not reserved or in use.
MCN
Basement - Electron Microscope (TEM) Not reserved or in use.
TEM Users
TEM USERS NOTE:
The CM-12 TEM has been removed and is being replaced during the week of 1/16-20 with the T-12 TEM.
Watch this site for updates on progress of the T-12 installation.

Training for the new system will be required.

Please call CISR for details: 2-5965 or 3-6691.
Don't Forget To Cite Us!
Acknowledgement for Cell Imaging Shared Resource

Our funding depends upon your citing the Cell Imaging Shared Resource when you publish data obtained with equipment or services from the CISR. This would include images from the microscopes, training in the use of software, prints from the Codonics, etc. Because we are fortunate to have so much Center support, the list of grant numbers is large.

Style I. The minimum acceptable acknowledgement should read:

"Experiments/Data analysis/presentation [include what you use]" were performed in part through the use of the VUMC Cell Imaging Shared Resource.

Style II. If space permits, please append the following list of grant numbers:

"Experiments/Data analysis/presentation [include what you use]" were performed in part through the use of the VUMC Cell Imaging Shared Resource (supported by NIH grants CA68485, DK20593, DK58404, HD15052, DK59637 and EY08126).

Please let us know when you publish, and if possible, send us a reprint of the paper.

This information is essential to sustain the imaging resources that you use.

Thank You.
New User Information
If you are interested in using the facility, here are some administrative as well as scientific issues to consider before you get started.

Click Here for more information and registration materials.
Tips and Tricks
Did you know the sample's surroundings will affect the image to a great degree. For optimal quality, you would ideally like your sample to be immersed in a material with the same index of refraction as our immersion oil (1.518).
Many people prefer to add specific anti-fade reagents, such as the following, to their mounting media (often made from scratch): 1,4-diazobicyclo-(2,2,2)octane (DABCO), n-propyl gallate, p-pheylenediamine dihydrochloride, or ascorbic acid.
Vanderbilt University Medical Center is the only Magnet Hospital in Metro Nashville area.
Did you know cold depolymerizes cytoskeletal elements, so whenever possible, buffer washes, fixatives, and sample should be at room temperature or warmer to initiate fixation.
All CISR microscopes are available ONLY to those who have been trained in their use by a member of the RESOURCE STAFF.
Vanderbilt University School of Medicine ranked 15th among all medical schools in total NIH awards, and 18th in US News & World Report in 2006.
The resource staff are happy to answer questions about our services and will help you plan your experiments. If you have questions please feel free to contact any member of the resource staff or the director.
EM Sectioning
All specimens for sectioning through the EM Resource must be accompanied by an EM work requisition and should be fixed prior to submission and kept refrigerated until submitted. Click Here to start or track your requsition.